Statement of Research Problem Sufian F. Alkhaldi, Ph.D.
DNA Microarray Lab.
Office of Regulatory Science (ORS), HFS-712
FDA/CFSAN -HFS712
5100 Paint Branch Parkway
College Park, MD 20740-3835
Tel: 301-436-1644 (office)
Tel: 301-436-2298 (lab)
Fax: 301-436-2632
E. mail: Sufian.AlKhaldi@fda.hhs.gov
Statement of Research
Problem
Current and future foodborne outbreaks require new technologies to provide fast and specific methods
for the isolation and detection of potentially dangerous pathogenic bacteria. Combining bacterial
isolation and identification technologies using automation will provide fast and high throughput screening abilities to rapidly identify
foodborne pathogenic bacteria. This work is focused on the requirements for the fast detection and response to natural and deliberate
microbiological food outbreaks, thus protecting the public health.
The objectives of my
research
(1) To develop a DNA chip for the identification of foodborne
bacteria.
(2) To develop novel label-free DNA chips for
bacterial gene detection by infrared micro-spectroscopy.
Some common techniques which are used in my lab: DNA
isolation, PCR amplification, Restriction enzyme analysis, DNA fluorescent PCR labeling, DNA Microarraying, Chip hybridization, Chip
fabrication, laser scanning, data analysis using bioinformatic programs, and many general molecular biology techniques.
|